Slaughter fish | Each fish is dissected and samples of liver, spleen, pyloric caeca, heart, kidney and encephalon are collected in culture medium as per the OIE Manual of Diagnostic Tests for Aquatic Animals. Organ samples from five fish are pooled into one sample. Samples are transported on ice to reach the Virology Section of the Onderstepoort Veterinary Institute (OVI) by the day following the day of sampling. Virus isolation takes place on RTG2, CHSE, and EPC cell lines.
Brood fish | Hens are stripped and a small number of ova with approximately 1 ml of ovarian fluid are collected from each fish and placed into culture medium. Samples from five fish are pooled into one sample and submitted to the OVI as per samples from slaughter fish.
Biosecurity measures and testing procedures at Sabieshoek Trout Farm were developed in the light of current knowledge of salmonid diseases and have been based on requirements laid down by the EU for certification of a disease free status for salmonids.